Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Phosphate Buffer, pH 8.0 is recommended for preparation of dilutions and blanks in accordance with USP.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Dibasic potassium phosphate | 16.730 |
| Monobasic potassium phosphate | 0.523 |
Final pH ( at 25°C) 8.0
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 17.25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.
Principle And Interpretation
Phosphate buffer pH 8.00 is formulated as described in USP (1). The phosphate buffer is required for the antibiotic preparation used in antibiotic assay.
Quality Control
Appearance White to cream homogeneous free flowing powder
Colour and Clarity of prepared medium Colourless clear solution without any precipitate
Reaction Reaction of 1.72% w/v aqueous solution at 25°C. pH : 8.0
pH 8.00
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- The United States Pharmacopoeia, 2009, The United States Pharmacopoeial Convention. Rockville, MD.
| Product Name | Phosphate Buffer, pH 8.0 |
|---|---|
| SKU | M1783 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. The United States Pharmacopoeia, 2009, The United States Pharmacopoeial Convention. Rockville, MD. |
| Customized Product Available | No |
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