Tryptone Soya Salt Agar w/ Magnesium Sulphate is recommended for enumeration of Vibrio parahaemolyticus from seafood by membrane filter technique as per APHA. The medium contains high salt concentration to meet requirement of Vibrio species from seafood. The medium is used after the seafood sample is diluted (1:10) with sterile Peptone Tween Salt Diluent (PTS), blended for 60 sec at high speed and filtered through Hydrophobic Grid Membrane Filter (HGMF).
1. Bruno Gomez-Gil and Ana Roque, Isolation, Enumeration and Preservation of the Vibrionaceae. F. L. Thompson, B. Austin and J. Swings. The Biology of Vibrios. ASM press.2.Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination ofFoods, 3rd Ed., APHA, Washington, D.C.3.Entis P. and Boleszczuk P., 1983, J. Food Prot., 46:783.