Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Gelatin Agar Slant
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$44.00
Availability: Out of stock
Availability: In stock
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SKU:
SL041
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Intended Use:
For cultivation and identification of members of Vibrio species.
Composition**
| Ingredients | g/L |
|---|---|
| Gelatin | 30.000 |
| Tryptone | 10.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions. Incubate the slants at 35-37°C for 24-48 hours.
Principle And Interpretation
Members of the genus Vibrio are facultative anaerobes capable of both respiratory and fermentative metabolism. The natural habitat for Vibrio species is aquatic, in both fresh water and salt water.
Gelatin Agar is formulated in accordance with APHA (1) for the cultivation and characterization of Vibrio species from foods and faeces. Clinical specimens must be obtained early in the disease as possible because the duration of excretion of the pathogen is short.
Type of specimen
Clinical sample- faeces; Food samples, Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection and processing as per guidelines (1).
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (2).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Gelatin Agar slant in glass tube.
Colour of medium
Yellow coloured medium
Quantity of medium
8ml of medium in glass tube
pH
7.00-7.40
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Gelatin liquefaction |
|---|---|---|---|
| Vibrio cholerae ATCC 15748 | 50-100 | luxuriant | positive reaction, clear zone around the colony within 24-48 hours |
| Vibrio parahaemolyticus ATCC 17802 (00037*) | 50-100 | luxuriant | positive reaction, clear zone around the colony within 24-48 hours |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Gelatin Agar Slant |
|---|---|
| SKU | SL041 |
| Customized Product Available | No |
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