Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Non Selective Media for Bacteria#CC293D
Intended Use
Recommended as a general purpose culture medium.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 5.000 |
| HM infusion B from ## | 150.000 |
| Peptone | 5.000 |
| Yeast autolysate | 1.500 |
| Sodium phosphate | 2.500 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Casein Hydrolysate
## Beef Infusion from
Directions
Suspend 35.5 grams in 1000 ml purified / distilled water containing 22 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour in sterile Petri plates.
Principle And Interpretation
Casitose Agar w/1.5% is the modification of medium recommended by APHA (6) and is used as a general purpose culture medium.
It has casitose, HM infusion B from, and peptone which serves as a rich source of nitrogen and carbon. Yeast autolysate provides necessary growth factors and vitamin supplement required for metabolism of wide number of bacteria. Sodium phosphate helps buffering of media whereas sodium chloride balances the osmotic equilibrium.
Type of Specimen
Food and dairy samples; Water samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,5,7).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2)
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitation
- 1.This medium is general purpose medium and may not support the growth of fastidious organisms.
- 2. Due to nutritional variations, some strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light to medium amber coloured, clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 3.55% w/v aqueous solution at 25°C. pH : 7.8 ± 0.2
pH: 7.60-8.00
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Casitose Agar w/ 1.5% Agar |
|---|---|
| SKU | M793 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C. |
| Customized Product Available | No |
Write Your Own Review
Nutrient Agar
As low as
₹0.00
Tryptone Glucose Extract Agar (Tryptone Glucose Yeast Extract Agar)
As low as
₹1,245.00
Upsell Products
Nutrient Agar Slant
As low as
₹1,139.00
Soyabean Casein Digest Agar Slant
As low as
₹1,139.00
Nutrient Agar
As low as
₹1,174.00
Soyabean Casein Digest Agar Plate (Tryptone Soya Agar Plate)
As low as
₹1,593.00