Phosphate Buffered Saline, pH 7.4 w/ 1gm/ litre Glucose and Phenol red w/o Calcium, Magnesium and Sodium bicarbonate

Phosphate Buffered Saline, pH 7.4 is most commonly used for tissue disaggregation and monolayer dispersal since presence of Calcium and Magnesium ions may hinder the trypsin activity. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation. Phosphate Buffered Saline, pH 7.4 contains 1gm per litre Glucose and Phenol red. It does not contain Calcium, Magnesium and Sodium bicarbonate.