HAT Medium Supplement 50X, Liquid w/ 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline

HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. Any unfused B-lymphocytes from the spleen cannot survive in culture for more than a few days. It is essential for B-cell-myeloma hybrids to contain the genetic information from both parent cells to enable them to survive in the HAT selection system. They can be cultured indefinitely and will produce unlimited quantities of antibody. After selection of the hybrid cells, the aminopterin from the medium is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. The hybrids are then transferred to a hybridoma growth medium. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.