Hi-PCR® Influenza Multiplex Probe PCR kit

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SKU:
MBPCR263
Influenza (commonly referred to as flu), a highly infectious disease known to cause an acute respiratory illness has afflicted humans since ancient times. Influenza viruses are a major cause of morbidity and mortality worldwide. The virus belongs to the family of Orthomyxoviridae that exist in three types, designated as influenza A, Influenza B and Influenza C. Influenza A virus infects humans and many other mammalian species including horses and swine and a wide variety of avian species while Influenza B virus naturally infects only humans. On the contrary, Influenza C virus rarely infects humans. In April 2009, a novel strain of swine origin influenza A H1N1 virus emerged in humans which caused a significant loss of lives across the globe.


Influenza A and Influenza B viruses are responsible for annual epidemics and irregular pandemics in humans. The grave threat posed by these emerging and re-emerging influenza viruses requires rapid laboratory detection. Real-time reverse-transcription polymerase chain reaction (RT-PCR) is considered as a gold standard method for influenza surveillance and diagnosis due to its high specificity, sensitivity and rapid detection. HiMedia has developed a Multiplex Reverse Transcriptase Real-Time PCR kit which enables the clinicians and public health laboratories to quickly diagnose influenza infection in a single tube.

NOTE: Hi-PCR® Influenza Multiplex Probe PCR Kit is for in-vitro use only.

Intended Use

Hi-PCR® Influenza Multiplex Probe PCR Kit is intended for use by qualified clinical laboratory personnel trained in the techniques of real-time PCR and in vitro diagnostic procedures. The kit is recommended for sensitive and specific detection of influenza virus in clinical samples.

Product Description

Hi-PCR® Influenza Multiplex Probe PCR Kit includes primer-probe sets specific to detect RNA from the three Influenza viruses: A, B & H1N1 pdm 09. In addition, an internal control (IC) for testing successful reactions. The Kit also provides synthetic positive controls for validity of the test.

Positive control

This is a control reaction using a known template (target pathogen). A positive control is usually used to ensure proper and intended functioning of all the reagents and is recommended to be used in every run to assess optimal performance.

Negative Control

A Negative control is needed to ensure that the reagents, equipment, and environment used in the assay is not contaminated with influenza RNA. In this reaction, Nuclease free water is used as the template. It is recommended to have minimum one reaction of negative control per run.

Internal Control

This is a control sequence that should amplify in all clinical samples which indicates the presence of sufficient RNA from human gene indicating the specimen is of acceptable quality. An internal control is often used to detect the failure of amplification in cases where the target sequence is not amplified.

Principle

Real-Time polymerase chain reaction, also called quantitative Polymerase Chain Reaction (qPCR) or kinetic Polymerase Chain Reaction, is a laboratory technique based on the principle of PCR. This technique is used to amplify a target DNA sequence by use of hydrolysis probes that are short oligonucleotides that have a fluorescent reporter dye attached to the 5' end and a quencher dye to the 3' end. This kit is designed to detect specific RNA targets from H1N1 pdm 09 virus in JOE channel, Influenza A virus (Inf A) in ROX channel and Influenza B virus (Inf B) in Cy5 channel. The internal control is FAM channel.
More Information
Product Name Hi-PCR® Influenza Multiplex Probe PCR kit
SKU MBPCR263
Customized Product Available No
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