Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Orange Serum HiVeg™ Broth
As low as
$146.30
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Availability: In stock
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SKU:
MV934
For cultivation of microorganisms associated with spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi.
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Intended Use
Orange Serum HiVeg Agar/Broth is used for cultivation and enumeration of microorganisms associated with the spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi.
Product Profile
Vegetable based (Code MV): MV933/MV934, HiVeg hydrolysate
Animal based (Code M): M933/M934, Casein enzymic hydrolysate
Recommended for
Cultivation and enumeration of microorganisms associated with the spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi.
Composition
| Ingredients | MV933 Grams/Litre | MV934 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 10.00 | 10.00 |
| Yeast extract | 3.00 | 3.00 |
| Dextrose | 4.00 | 4.00 |
| Dipotassium phosphate | 2.50 | 2.50 |
| Orange serum (solids from 200 ml) | 9.00 | 9.00 |
| Agar | 17.00 | - |
Final pH (at 25°C) 5.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Reconstitution Information
Reconstitution:
- (MV933): 45.5 g/l
- (MV934): 28.5 g/l
Quantity on preparation (500g):
- (MV933): 10.98 L
- (MV934): 17.54 L
pH (25°C): 5.5 ± 0.2
Supplement: None
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium & Prepared Medium 2 - 8°C.
Directions
Suspend 45.5 grams of MV933 or 28.5 grams of MV934 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.
Principle and Interpretation
These media are developed by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptones. Orange Serum Agar was originally developed by Murdock et al (1) and Hays (2) for examining citrus concentrates. Hays and Reister further used this medium for studying spoilage of orange juice (3). Murdock and Brokaw (4) employed Orange Serum Broth for studies of sanitary control of the processing of citrus concentrates. Hays and Reister recommended Orange Serum Broth, pH 5.5 which is accepted as a control medium by the Citrus Industry since at this reaction, the medium is most productive for the growth of spoilage organisms. Dehydrated agar media containing orange serum was reported by Stevens (5) and these media are recommended by APHA (6). Orange Serum HiVeg media are the modification of these media and serves the same purpose. HiVeg hydrolsate and yeast extract provide essential nitrogenous nutrients while dextrose serves as the energy source. Orange serum gives an optimal environment for the recovery of acid tolerant microorganisms from citrus fruit products.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.7% Agar gel of MV933.
Colour and Clarity: Medium to dark amber coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction: Reaction of 4.55% w/v of MV933 or 2.85% w/v of MV934 aqueous solution is pH 5.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| *Aspergillus niger (16404) | good-luxuriant |
| Lactobacillus fermentum (9338) | good-luxuriant |
| Lactobacillus acidophilus (4356) | good-luxuriant |
| Leuconostoc mesenteroides (12291) | good-luxuriant |
| Saccharomyces ceresivisiae (9763) | good-luxuriant |
Key: * = Incubated at 25-30°C.
| Product Name | Orange Serum HiVeg™ Broth |
|---|---|
| SKU | MV934 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.2.Murdock P. I., Folinazzo J. F., and Troy V. S., 1951, Food Technol., 6:181.3.Stevens J. W., 1954, Food Technol., 8:88.4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore5.Murdock P. I. and Brokaw C. H., 1958, Food Technol., 12:573. |
| Customized Product Available | No |
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Orange Serum Broth
As low as
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