Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Vibrio HiVeg™ Agar
As low as
$254.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV820
Used for selective cultivation of Vibrio species.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| Yeast extract | 5.0 |
| HiVeg hydrolysate | 8.0 |
| HiVeg peptone No. 3 | 3.0 |
| Sucrose | 20.0 |
| Sodium thiosulphate, 5H2O | 6.5 |
| Sodium citrate. 2H2O | 10.0 |
| Synthetic detergent No. III | 1.0 |
| Sodium chloride | 10.0 |
| Synthetic detergent No. II | 1.0 |
| Sodium lauryl sulphate | 0.2 |
| China blue | 0.2 |
| Cresol red | 0.2 |
| Agar | 15.0 |
| Final pH (at 25°C) | 8.5 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Data
Vegetable based (Code MV)®
- MV820
- HiVeg hydrolysate
- HiVeg peptone No. 3
- Synthetic detergent No. II
- Synthetic detergent No. III
Animal based (Code M)
- M820
- Casein enzymic hydrolysate
- Proteose Peptone
- Oxgall
- Sodium deoxycholate
Recommended for: Selective cultivation of Vibrio species.
| Reconstitution | 74.84 g/l |
| Quantity on preparation (500g) | 6.68 L |
| pH (25°C) | 8.5 ± 0.2 |
| Supplement | None |
| Sterilization | Boiling (DO NOT AUTOCLAVE) |
Storage: Dry Medium-Below 30°C, Prepared Medium 2-8°C.
Directions
Suspend 74.84 grams of dehydrated medium in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45 - 50°C and pour into sterile petri plates.
Principle and Interpretation
Vibrio HiVeg Agar is prepared by using vegetable peptones in place of animal based peptones that makes the medium free of BSE/TSE risks. Vibrio HiVeg Agar is the modification of Vibrio Agar which is a selective medium for the isolation of Vibrio cholerae, Vibrio parahaemolyticus as well as other Vibrios (1).
HiVeg hydrolysate, HiVeg peptone No.3 and yeast extract provide nitrogenous, carbonaceous compounds, sulphur, vitamin B complex and other essential growth nutrients. Sodium citrate, and synthetic detergents inhibit gram-positive organisms and coliforms. Sucrose is the fermentable carbohydrate while thiosulphate acts as a sulphur source. Alkaline pH of this medium helps in recovery of Vibrio cholerae. China blue and cresol red are pH indicators.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis (29212) | 102-103 | none-poor | <20% | yellow |
| Escherichia coli (25922) | 102-103 | inhibited | 0% | - |
| Pseudomonas aeruginosa (27853) | 102-103 | none-poor | <20% | blue |
| Salmonella serotype Typhi (6539) | 102-103 | inhibited | 0% | - |
| Shigella flexneri (12022) | 102-103 | inhibited | 0% | - |
| Vibrio cholerae (15748) | 102-103 | good-luxuriant | >50% | blue |
| Vibrio parahaemolyticus (17802) | 102-103 | good-luxuriant | >50% | slightly reddish |
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Reddish purple coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 7.48% w/v aqueous solution is pH 8.5 ± 0.2 at 25°C.
References
- Atlas, R.M. 1993, Handbook of Microbiological Media, Parks, L.C. (Ed.), CRC Press, Boca Raton.
| Product Name | Vibrio HiVeg™ Agar |
|---|---|
| SKU | MV820 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M. 2004, 3rd Ed., Handbook of Microbiological Media, Parks, L.C., (Ed.), CRC Press, Boca Raton. |
| Customized Product Available | No |
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