Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Sulphate Reducing HiVeg™ Medium (Triple Pack)
As low as
$51.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV803
Recommended for enumeration of sulphate reducing bacteria in water samples.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Part A: | |
| HiVeg peptone | 2.0 |
| HiVeg extract | 1.0 |
| Magnesium sulphate. 7H₂O | 2.0 |
| Sodium sulphate | 1.5 |
| Dipotassium phosphate | 0.5 |
| Calcium chloride | 0.1 |
| Part B: | |
| Ferrous ammonium sulphate. 6H₂O | 0.392 |
| Sodium ascorbate | 0.1 |
| Part C: | |
| Sodium lactate | 3.5 |
| Final pH (at 25°C) | 7.5 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | |
| MV803 | M803 | |
| HiVeg peptone | Peptic digest of animal tissue | |
| HiVeg extract | Beef extract | |
| Recommended for | : | Enumeration of sulphate reducing bacteria in water samples. |
| Reconstitution | : | 6.07 g/l of Part A + 0.492 g/l of Part B + 3.5 g/l of Part C |
| Quantity on preparation (500g) | : | 49.69 L (A+B+C) |
| (100g) | : | 9.93 L (A+B+C) |
| pH (25°C) | : | (Part A) : 7.5 ± 0.2 |
| Supplement | : | None |
| Sterilization | : | (Part A & C) : 121°C / 15 minutes |
| : | (Part B) : Filtration | |
| Storage | : | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 6.07 grams of dehydrated medium of Part A in 900 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. On the day of use prepare solution of Part B in 100 ml distilled water. Sterilize by filtration through a 0.45 µm membrane filter and aseptically add this 100 ml solution to 900 ml Part A medium. Then separately sterilize the Part C by autoclaving at 15 lbs pressure (121°C) for 15 minutes and aseptically add to the mixture of Part A and B. Mix well and aseptically transfer the complete medium to sterile screw capped tubes filling them completely.
Principle and Interpretation
Sulphate Reducing HiVeg Medium is prepared by using vegetable peptones in place of animal based peptones which makes the medium free from BSE/TSE risks. Sulphate Reducing HiVeg Medium is the modification of Sulphate Reducing Medium which is formulated in accordance with APHA (1) for enumeration of sulphate reducing bacteria. Sulphate reducing bacteria such as Desulfovibrio converts sulphate to sulphide which reacts with ferrous ions to give a black colour within 4 to 21 days at 20-30°C.
The tubes are filled completely to create anaerobic conditions. When sample volume is greater than 10 ml, sample is passed through a 0.45 µm membrane filter and the filter is transferred to screw-capped test tubes containing medium, and observed for colour change.
Quality Control
Appearance of powder
- Part A: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
- Part B: Creamish white coloured, homogeneous, free flowing powder.
- Part C: Colourless solution.
Colour and Clarity
Light yellow coloured, clear to slightly opalescent solution in tubes.
Reaction
Reaction of 0.61grams in 90 ml aqueous solution of Part A is pH 7.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 20-30°C for upto 4-21 days.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Desulfovibrio desulfuricans (29577) | 10²-10³ | luxuriant |
| Thiobacillus thioxidans (19377) | 10²-10³ | good-luxuriant |
| Product Name | Sulphate Reducing HiVeg™ Medium (Triple Pack) |
|---|---|
| SKU | MV803 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2012, Standard Methods for the Examination of Water and Wastewater, 23rd Ed., APHA, Washington, D.C. |
| Customized Product Available | No |
Write Your Own Review
Starkey's Sulphate Reducing Agar Base
As low as
$163.90
Sulphate Reducing Medium (Twin Pack)
As low as
$51.70
Sulphate Reducing Medium (Triple Pack)
As low as
$51.70
Upsell Products
HiDip™ Sulphate API - PCA Medium
As low as
$28.60
HiDip™ Sulphate API-Sulphate API
As low as
$36.30