Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Sulphate Reducing Medium (Triple Pack)
As low as
$51.70
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Availability: In stock
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SKU:
M803
Recommended for enumeration of sulphate reducing bacteria in water samples.
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Intended use
Sulphate Reducing Medium is recommended for enumeration of sulphate reducing bacteria in water samples.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Part A | |
| Dipotassium phosphate | 0.500 |
| Peptone | 2.000 |
| Meat extract B# | 1.000 |
| Sodium sulphate | 1.500 |
| Magnesium sulphate, heptahydrate | 2.000 |
| Calcium chloride | 0.100 |
| Part B | |
| Ferric ammonium sulphate, hexahydrate | 0.392 |
| Sodium ascorbate | 0.100 |
| Part C | |
| Sodium lactate | 3.500 |
| Final pH (at 25°C) | 7.5±0.3 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 6.08 grams(equivalent weight of dehydrated media per litre) of Part A in 900 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. On the day of use prepare solution of Part B by suspending 0.384 grams(equivalent weight of dehydrated media per litre) of Part B in 100 ml distilled water. Sterilize by filtration through a 0.45 µm membrane filter and aseptically add this 100 ml solution to 900 ml Part A medium. Then separately sterilize the 3.50 grams Part C by autoclaving at 15 lbs pressure (121°C) for 15 minutes and aseptically add to the mixture of Part A and B. Mix well and aseptically transfer the complete medium to sterile screw capped tubes filling them completely.
Principle And Interpretation
Sulphate Reducing Medium is formulated in accordance with APHA (1) for enumeration of sulphate reducing bacteria. Sulphate reducing bacteria such as Desulfovibrio converts sulphate to sulphide which reacts with ferrous ions to give a black colour within 4 to 21 days at 20-30°C. Thiobacillus also produces sulphuric acid and hence is found in environment containing H2S (2). Peptone and meat extract B in the medium provide nitrogen and other nutrients necessary to support bacterial growth. Potassium phosphates buffer the medium. Sodium chloride and the sulphate salts provide essential ions. The tubes are filled completely to create anaerobic conditions. When sample volume is greater than 10 ml, sample is passed through a 0.45 um membrane filter and the filter is transferred to screw-capped test tubes containing medium.
Type of specimen
Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidleines should be followed while handling clincal specimens. Saftey guidelines may be referred in individual safety data sheets
Limitations
- Longer incubation period may be required for isolates.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance of Part A
Part A: Cream to yellow homogeneous free flowing powder.
Part B: White to cream homogeneous free flowing powder.
Part C: Colourless solution
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution in tubes
Reaction
Reaction of 0.62% w/v Part A + 0.0384% w/v Part B + 0.35% v/v Part C aqueous solution at 25°C. pH: 7.5±0.3
pH
7.20-7.80
Cultural Response
Cultural characteristics observed after an incubation at 20-30°C for upto 4-21 days.
Cultural Response
| Organism | Growth |
|---|---|
| Desulfovibrio desulfuricans ATCC 13541 | luxuriant |
| Thiobacillus thiooxidans ATCC 19377 | good-luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Sulphate Reducing Medium (Triple Pack) |
|---|---|
| SKU | M803 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2012, Standard Methods for the Examination of Water and Wastewater, 23rd Ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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