Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Halophilic HiVeg™ Broth
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$255.20
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SKU:
MV591
For the isolation and cultivation of extremely halophilic bacteria.
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Intended Use
Halophilic HiVeg Agar / Broth is used for the isolation and cultivation of extremely halophilic bacteria.
Product Profile
Vegetable based (Code MV)
- MV590/MV591
- HiVeg acid hydrolysate
- HiVeg peptone No. 3
Animal based (Code M)
- M590/M591
- Casein acid hydrolysate
- Proteose peptone
| Recommended for | : Isolation and cultivation of extremely halophilic bacteria. |
| Reconstitution | : (MV590): 325.0 g/l |
| : (MV591): 305.0 g/l | |
| Quantity on preparation (100g) | : (MV590): 0.30 L |
| : (MV591): 0.32 L | |
| pH (25°C) | : 7.2 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes. |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Composition
| Ingredients | MV590 Grams/Litre | MV591 Grams/Litre |
|---|---|---|
| HiVeg acid hydrolysate | 10.00 | 10.00 |
| Yeast extract | 10.00 | 10.00 |
| HiVeg peptone No.3 | 5.00 | 5.00 |
| Trisodium citrate | 3.00 | 3.00 |
| Potassium chloride | 2.00 | 2.00 |
| Magnesium sulphate | 25.00 | 25.00 |
| Sodium chloride | 250.00 | 250.00 |
| Agar | 20.00 | — |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 32.5 grams of MV590 or 30.5 grams of MV591 in 100 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
These media are prepared by completely replacing animal based peptone by vegetable peptones which is free from BSE/TSE risks. Halophilic HiVeg media are the modification of Halophilic media which are formulated for isolation and cultivation of extreme halophilic species of Halobacterium and Halococcus from foods (1, 2). For optimum growth they require high salt concentration of about 20 - 30%.
These bacteria can cause pink discoloration on the outer surface accompanied by putrefaction and decomposition of fish, bacon and hides preserved in sea salts.
Halophilic media contains HiVeg acid hydrolysate, HiVeg peptone No.3 and yeast extract which provide all the necessary nutrients, mainly nitrogenous and vitamins to the halophilic bacteria. Trisodium citrate serves as selective agent and prevents loss of halophiles in mixed population; as it supresses Gram positive organisms and coliforms (2).
Magnesium is an essential ion for the growth of extreme halophiles and is incorporated in the medium as magnesium sulphate.
10 gm sample is added to 90 ml Halophilic HiVeg Broth and incubated at 35°C for upto 12 days. The organisms are then isolated onto Halophilic HiVeg Agar from this enriched culture.
Quality Control
Appearance of Powder
Off white coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 2.0% Agar gel of MV590.
Colour and Clarity
Amber coloured, slightly opalescent gel forms in petri plates, hazy solution containing heavy precipitate at the bottom in tubes.
Reaction
Reaction of 32.5% w/v of MV590 or 30.5% w/v of MV591 aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 12 days.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Halococcus morrhuae (17082) | 102-103 | luxuriant | >70% |
| Halobacterium salinarium (33171) | 102-103 | luxuriant | >70% |
References
- Dundas I.E., 1977, Advances In Microbiology and Physiology, Rose H. and Tempest D.W. (Eds.), A.P. London.
- Gibbons N.E., 1969, Methods In Microbiology, Vol. 3B, Norris J.R., and Ribbons D.W. (Eds.), A.P., New York, pp.169-183.
| Product Name | Halophilic HiVeg™ Broth |
|---|---|
| SKU | MV591 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Dundas I.E., 1977, Advances In Microbiology and Physiology, Rose H. and Tempest D.W. (Eds.), A.P. London. 2.Gibbons N.E., 1969, Methods In Microbiology, Vol. 3B, Norris J.R., and Ribbons D.W. (Eds.), A.P., New York, pp.169-183. 3.Kushner D. J., (Eds.), 1978, D. J. Kushner, pg 317, Academic Press, London, England 4.MacLeod R. A., 1965, Bacteriol., Rev., 29:9 |
| Customized Product Available | No |
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Halophilic Broth
As low as
$255.20