Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Peptone Iron HiVeg™ Agar
As low as
$88.00
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV440
For detection of hydrogen sulfide production by microorganisms.
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Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV440 | M440 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg peptone No. 3 | Proteose Peptone |
- Recommended for
- Detection of hydrogen sulfide production by microorganisms
- Reconstitution
- 36.58 g/l
- Quantity on preparation (100g)
- 2.73 L
- pH (25°C)
- 6.7 ± 0.2
- Supplement
- None
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 15.0 |
| HiVeg peptone No. 3 | 5.0 |
| Ferric ammonium citrate | 0.5 |
| Sodium glycerophosphate | 1.0 |
| Sodium thiosulphate | 0.08 |
| Agar | 15.0 |
| Final pH (at 25°C) | 6.7 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 36.58 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to cool in an upright position.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg peptone No.3 which are free from BSE/TSE risks associated with animal based peptones. Peptone Iron HiVeg Agar is the modification of formulation described by Levine et al (1,2) This medium contains HiVeg peptones and ferric ammonium citrate is used for the detection of H₂S (hydrogen sulphide) production by microorganisms. This medium is helpful in differentiating the strains which are Voges-Proskauer negative, methyl red positive and citrate positive from the other strains of Enterobacteriaceae family.
Ferric ammonium citrate is a better hydrogen sulphide indicator as compared to lead acetate, as it gives earlier and clearer results.
HiVeg peptone and HiVeg peptone No.3 provide nitrogenous compounds, sulphur and trace elements. Sodium thiosulphate and ferric ammonium citrate forms the hydrogen sulphide detecting system. Sodium glycerophosphate buffers the medium.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light amber coloured, slightly opalescent gel forms in tubes.
Reaction
Reaction of 3.66% w/v aqueous solution is pH 6.7 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | H₂S production |
|---|---|---|---|---|
| Enterobacter aerogenes (13048) | 10²-10³ | luxuriant | >70% | - |
| Escherichia coli (25922) | 10²-10³ | luxuriant | >70% | - |
| Salmonella serotype Typhi (6539) | 10²-10³ | luxuriant | >70% | + |
| Salmonella serotype Enteritidis (13076) | 10²-10³ | luxuriant | >70% | + |
Key : + = blackening of the medium
- = no colour change
| Product Name | Peptone Iron HiVeg™ Agar |
|---|---|
| SKU | MV440 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Koneman E. W, Allen S. D., Janda W. M., Schreckenberger P. C., Winn W.C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4th Ed, J. B. Lippincott Company, Philadelphia. 2.Levine M., Vaughn R., Epstein S. S. and Anderson D., 1932, Proc. Soc. Exp. Biol. Med. 29:1022. 3.Levine M., Epstein S. S. and Vaughn R., 1934, Am. J. Public Health 24 :505. 4.Tittsler R. P. and Sandholzer L. A., 1937, Am. J. Public Health 27:1240. |
| Customized Product Available | No |
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