Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Eugonic HiVeg™ Agar
As low as
$210.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV428
For the cultivation of fastidious microorganisms like Haemophillus, Neisseria, Posteurella,Brucella and Lactobacillus species.
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Intended Use:
Recommended for the cultivation of fastidious microorganisms like Haemophillus, Neisseria, Pasteurella, Brucella and Lactobacillus species from various samples.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 15.000 |
| Soya peptone | 5.000 |
| Dextrose (Glucose) | 5.000 |
| Sodium chloride | 4.000 |
| Sodium sulphite | 0.200 |
| L-Cystine | 0.200 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 44.4 grams in 1000 ml purified/distilled water. Heat to boiling with frequent stirring to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Cool to 45-50°C and add 5 -10% v/v sterile defibrinated blood if desired. The blood may be chocolated by heating, if chocolate agar plates are required. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Eugonic Agar was developed by Pelczar and Vera (1) for cultivation of fastidious organisms like Brucella. These media can also be used to grow Mycobacteria and various pathogenic fungi including Nocardia, Histoplasma and Blastomyces, when enriched with blood. Niven used this media for detection of spoilage of meats (2). Eugonic Agar was developed to obtain eugonic (luxuriant) growth of fastidious microorganisms like Brucella that are otherwise difficult to cultivate (3). The unnourished medium supports rapid growth of lactobacilli associated with cured meat products, dairy products and other foods. APHA recommends Eugonic agar, which is also used in germinating anaerobic spores pasteurized at 104°C (4,5). Eugonic HiVeg™ Agar is quite similar to Tryptone Soya HiVeg™ Agar (MV290) but more bacterial propagation is expected on Eugonic HiVeg™ Agar. Organisms like Bordetella and Neisseria form minute colonies on Tryptone Soya HiVeg™ Agar (MV290). They may become large on Eugonic HiVeg™ Agar because large amount of sulfur and carbon sources have been added in addition to the Tryptone Soya HiVeg™ Agar (MV290) formula. Therefore this medium is recommended for the direct isolation of Bordetella pertussis and Neisseria meningitides from the test materials such as throat mucus, blood, cerebrospinal fluid, pleural fluid and other specimens. For the isolation of Bacillus pumilus, Eugonic Agar can be supplemented with 0.1% starch, prior to sterilization (3). Eugonic HiVeg™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate and soya peptone provide the nitrogen, vitamins and amino acids, which supports the growth of fastidious microbial species. The high concentration of dextrose is the energy source for rapid growth of bacteria. L-Cystine and sodium sulphite are added to stimulate growth. Sodium chloride maintains the osmotic balance of the media. The high carbohydrate content along with high sulfur (cystine) content improves growth with chromogenicity (4).
Type of specimen
Food samples
Specimen Collection and Handling:
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Due to the high carbohydrate content, medium should not be used to detect ß-hemolysis. (2)
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.44% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed with added 5-10% sterile defibrinated blood after an incubation at 35-37°C for 48 hours (fungal cultures incubated at 25-30°C).
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus pumilus ATCC 14884 | 50-100 | good (with 0.1% starch) | 50-70% |
| Candida albicans ATCC 26790 | 50-100 | good | 50-70% |
| Lactobacillus fermentum ATCC 9338 | 50-100 | good | 50-70% |
| Neisseria meningitidis ATCC 13090 | 50-100 | good | 50-70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant(under >=70% 3-5% CO2) | |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant(under >=70% 3-5% CO2) |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Eugonic HiVeg™ Agar |
|---|---|
| SKU | MV428 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Pelczar and Vera J., 1949, Milk Plant Monthly 38:30 2.Niven C. F., Castellani A. G., and Allanson V., 1949, J. Bacteriol., 58:633. 3.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C. 4.Frank H. A., 1955, J. Bacteriol., 70:269. 5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams& Wilkins, Baltimore, Md. |
| Customized Product Available | No |
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