Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Eugonic LT100 Broth
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$73.70
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SKU:
LQ208
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Intended use
Recommended for activation of fastidious organisms.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Glucose (Dextrose) | 5.500 |
| Sodium chloride | 4.000 |
| Sodium sulphite | 0.200 |
| L-Cystine | 0.700 |
| Egg lecithin | 1.000 |
| Triton X-100 | 1.000 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ208 bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle and Interpretation
Eugonic LT 100 Medium Base was developed by Pelczar and Vera (1) for cultivation of fastidious organisms like Brucella. Eugon media were developed to obtain eugonic (luxuriant) growth of fastidious microorganisms like Brucella which are otherwise difficult to cultivate (2). The unenriched medium supports rapid growth of lactobacilli associated with cured meat products, dairy products and other foods. Eugonic media is quite similar to Tryptone Soya Agar (MP290) but more bacterial propagation is expected on Eugonic media. Organisms like Bordetella and Neisseria form minute colonies on Tryptone Soya Agar (MP290). They may become large on Eugon Media because large amount of sulfur and carbon sources have been added in addition to the Tryptone Soya Agar (MP290) formula. The composition of the medium is as per ISO (3) for the detection of mesophilic aerobic bacteria from cosmetic products.
Tryptone and soya peptone provide the nitrogen, carbon compounds, vitamins and amino acids, which supports the growth of fastidious microbial species. The high concentration of glucose is the energy source for rapid growth of bacteria. L-Cystine and sodium sulphite are added to stimulate growth. Sodium chloride maintains the osmotic balance of the media. The high carbohydrate content along with high sulfur (cystine) content improves growth with chromogenicity Lecithin and polysorbate 80 in Eugonic LT 100 Broth neutralize antimicrobial agents hence this medium can be used as a neutralizing diluent (4).
Type of specimen
Clinical samples - urine, faeces, Cosmetic samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6). For cosmetic samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Further biochemical and serological tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear Eugonic LT 100 Broth in glass bottle.
Colour
Yellow coloured clear solution
Quantity of Medium
10ml of medium in glass bottle.
pH
6.80-7.20
Sterility Test
Passes release criteria
Cultural Response :
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours (fungal cultures incubated at 25-30°C for 2-7 days).
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Bacillus pumilus ATCC 14884 | 50-100 | good |
| Candida albicans ATCC 26790 | 50-100 | good |
| Lactobacillus fermentum ATCC 9338 | 50-100 | good |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant (under 3-5% CO2) |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant (under 3-5% CO2) |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp.aureus ATCC 6538 (00032*) | 50-100 | good |
| **Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | good |
| ^Pseudomonas paraaeruginosa ATCC 9027 (00026*) | 50-100 | good |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | good |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good |
| Neisseria meningitidis ATCC 13090 | 50-100 | good |
Key: (*) Corresponding WDCM Numbers
^ Formerly known as Pseudomonas aeruginosa **Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Eugonic LT100 Broth |
|---|---|
| SKU | LQ208 |
| Customized Product Available | No |
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