Skip to the beginning of the images gallery

Lysine Decarboxylase HiVeg™ Broth

0 Review

As low as $48.40

Availability: Out of stock

Notify me when the price drops User

Availability: In stock

Only %1 left

SKU:

MV376

For differentiating Salmonella arizonae from the Bethesda Ballerup group of Enterobacteriaceae.

Description

Intended Use

Lysine Decarboxylase HiVeg Broth is used for distinguishing Salmonella serotype Arizonae from the Bethesda Ballerup group of Enterobacteriaceae.

Product Profile

Vegetable based (Code MV)	Animal based (Code M)
MV376	M376
HiVeg peptone	Peptic digest of animal tissue

Composition

Ingredients	Grams/Litre
HiVeg peptone	5.0
Yeast extract	3.0
Dextrose	1.0
L-Lysine hydrochloride	5.0
Bromo cresol purple	0.02

Final pH (at 25°C) 6.8 ± 0.2

Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 14 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense 5 ml amount into screw capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle and Interpretation

This medium is prepared by completely replacing animal based peptones with vegetable peptones. Lysine Decarboxylase HiVeg Broth is the modification of Lysine Decarboxylase Broth which was originally formulated by Falkow (1).

During the initial stages of incubation, fermentation of glucose by the organisms, yields acid production resulting in a colour change of indicator to yellow. On further incubation, if L-Lysine is decarboxylated to cadaverine, there will be an alkaline reaction and indicator colour will then change to purple. If colour remains yellow, the decarboxylase reaction is negative. HiVeg peptone and yeast extract provide essential growth nutrients. Dextrose is the fermentable carbohydrate and bromo cresol purple is the pH indicator. Use light inocula and do not read the tests under 24 hours incubation as some organisms require longer incubation time upto 4 days. To obtain proper reactions, inoculated tubes must be protected from air. This is done to avoid false alkalinization at the surface of the medium, which could cause a decarboxylase negative bacteria to appear to be positive. This can be done by overlaying a medium with sterile mineral oil as suggested by Ewing, Davis and Edwards (2).

Quality Control

Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity: Purple coloured, clear solution without any precipitate.

Technical Data

Reconstitution

Quantity on preparation (500g): 35.71 L
(100g): 7.14 L

Supplement

None

Sterilization

121°C / 15 minutes.

Storage

Dry Medium Below 30°C, Prepared Medium 2-8°C.

Reaction

Reaction of 1.4% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 18-24 hours.

Organisms (ATCC)

Organisms (ATCC)	Inoculum (CFU)	Lysine decarboxylation*
Citrobacter freundii (8090)	10²-10³	@
**Enterobacter aerogenes (13048)	10²-10³	+
Escherichia coli (25922)	10²-10³	±
#Klebsiella pneumoniae (13883)	10²-10³	+
Proteus mirabilis (25933)	10²-10³	-
Proteus vulgaris (13315)	10²-10³	-
Salmonella serotype Arizonae (13314)	10²-10³	+
Salmonella serotype Paratyphi A (5006)	10²-10³	-
Salmonella serotype Typhi (6539)	10²-10³	+
Serratia marcescens (8100)	10²-10³	+
Shigella dysenteriae (13313)	10²-10³	-

Key

+ = positive reaction, purple colour
- = negative reaction, yellow colour or no change
* = inoculated tubes overlaid with Sterile Mineral oil.
± = variable
@ = it is negative but shows false positivity
** = including the Bethesda-Ballerup group
# = including late lactose variants Alkalescens - Dispar

Product Information

More Information
Product Name	Lysine Decarboxylase HiVeg™ Broth
SKU	MV376
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rd ed., APHA, Washington, D.C. 2.Eaton A. D., Clesceri L. S., Rice E. W. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water and Wastewater, 21st Ed., APHA, Washington, D.C. 3.FDA Bacteriological Analytical Manual, 2017, AOAC, Washington, DC. 4.Falkow, 1958, Am. J. Clin. Pathol., 29:598. 5.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 6.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1 7.Moeller V., 1954, Acta. Pathol. Microbiol. Scand., 34:102. 8.Moeller V., 1954, Acta. Pathol. Microbiol. Scand., 34:259.9.Moeller V., 1955, Acta. Pathol. Microbiol. Scand., 36:158 10.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods,5th Ed., American Public Health Association, Washington, D.C
Customized Product Available	No