Lysine Decarboxylase Broth w/o Peptone

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M376I
for distinguishing the Salmonella arizonae from the Bethesda Ballerup group of Enterobacteriaceae. The composition and performance criteria are in accordance with ISO 6579-1:2017.


Lysine Decarboxylase Broth is recommended for differentiating Salmonella arizonae from the Bethesda Ballerup group of Enterobacteriaceae. The composition and performance criteria are in accordance with ISO 6579-1:2017.Fermentation of dextrose by the organisms leads to acid production, which causes a subsequent colour change of the bromocresol purple indicator to yellow. The acidic condition thus generated stimulates decarboxylase activity, which leads to decarboxylation of lysine to cadaverine. The alkaline conditions generated due to cadaverine production cause the bromocresol purple indicator (changed to yellow) to revert to purple colour. If the organisms do not produce decarboxylase enzyme, the colour of the medium remains yellow. Salmonella Arizonae, Salmonella Typhi, Klebsiella pneumoniae, Klebsiella aerogenes are known to decarboxylate lysine and show positive reaction.
More Information
Product Name Lysine Decarboxylase Broth w/o Peptone
SKU M376I
Product Type Regular
Physical Form Powder
Origin Animal Free (Microbial)
Packaging type HDPE
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2.Moeller V., 1954, Acta. Pathol. Microbiol. Scand., 34:259.
3.Moeller V., 1955, Acta. Pathol. Microbiol. Scand., 36:158.
4.Falkow, 1958, Am. J. Clin. Pathol., 29:598.
5.Eaton A. D., Clesceri L. S., Rice E. W. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Waterand Wastewater, 21st Ed., APHA, Washington, D.C.
6.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.
7.Isenberg (Ed.), 1992, Clinical Microbiology Procedures Handbook, Vol. 1. American Society for Microbiology, Washington,D.C.
8.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.
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