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EC Blue HiVeg™ Broth

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MV1768

For detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.

Description

EC Blue HiVeg™Broth is recommended for detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.Escherichia coli can be distinguished from other coliforms by its unique ability to fluoresce in the presence of fluorogenic substrate. The fluorogenic substrate, MUG is split by enzyme β-glucuronidase especially present in Escherichia coli. The reaction is indicated by the development of a blue fluorescence under UV light. The presence of total coliforms is indicated by blue-green colourations due to the cleavage of the chromogenic substrate (X-Gal). IPTG amplifies enzyme synthesis and increases the activity of β -galactosidase. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk.

Product Information

More Information
Product Name	EC Blue HiVeg™ Broth
SKU	MV1768
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C.2.Feng P.C.S. and Hartman P.A. ,1982, J.Appl. Environmental Microbiol. 43.1320-1323.3.Harsen W., and Yourassowsky, 1984, J.Clin. Microbiol.20.1177-1179.4.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.5.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. 6.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.7.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
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