Recommended for the simultaneous detection of Escherichia coli and total coliforms in water and food based on chromogenic differentiation. It contains two chromogenic substrates. The enzyme ß-D-galactosidase produced by coliforms cleaves one of the chromogen to form salmon to red coloured colonies (5). The enzyme produced by E.coli, cleaves X-glucuronide, the other chromogen. Colonies of E.coli gives dark blue to violet coloured due to presence of ß-D-glucuronidase and ß-D-galactosidase. On addition, on Kovacs reagent, formation of cherry red colour indicates a positive indole reaction. Sterile pad is supplied in sterile Petri plate. Membrane filter is not supplied .