Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Potato Infusion HiVeg™ Agar
As low as
$251.90
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Availability: In stock
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SKU:
MV174
Recommended for the isolation of Brucella species.
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Intended Use
Potato Infusion HiVeg Agar is recommended for isolation of Brucella species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Potato infusion from | 200.00 |
| HiVeg peptone | 10.0 |
| HiVeg extract | 5.0 |
| Dextrose | 10.0 |
| Sodium chloride | 5.0 |
| Agar | 15.0 |
| Final pH (at 25°C) | 6.8 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 49 grams in 1000 ml of distilled water containing 20 ml of glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Potato Infusion HiVeg Agar is prepared by replacing Peptic digest of animal tissue and Beef extract with HiVeg peptone and HiVeg extract which are free from BSE/TSE risks. Potato Infusion HiVeg Agar is the modification of Potato Infusion Agar which is used for the isolation of Brucella species. Brucella causes zoonotic diseases with a domestic animal reservoir (1). Transmission by milk, milk products, meat and direct contact with infected animals is the usual route of exposure. This medium contains infusion of potato, HiVeg extract and HiVeg peptone which provide necessary nutrients required for the growth of Brucella. It is also used for the cultivation of Brucella species in large scale in the antigen and vaccine preparation. This medium enables Brucella species to form typical colonies and is also used for isolation from infected materials.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV174 | M174 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Technical Data
| Recommended for | Isolation of Brucella species |
| Reconstitution | 49.0 g/l |
| Quantity on preparation (500g) | 10.20 L |
| pH (25°C) | 6.8 ± 0.2 |
| Supplement | Glycerol |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Yellow coloured clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 4.9% w/v aqueous solution (containing 2% v/v glycerol) is pH 6.8 ± 0.2 at 25°C
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24 - 72 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Bordetella bronchiseptica (4617) | 102-103 | luxuriant |
| Brucella abortus (4315) | 102-103 | luxuriant |
| Brucella melitensis (4309) | 102-103 | luxuriant |
| Brucella suis (6597) | 102-103 | luxuriant |
| Streptococcus pneumoniae (6303) | 102-103 | luxuriant |
References
- Murray PR, Baron, Pfaller and Yolken (Eds), 2003, In Manual of Clinical Microbiology, 8th Ed, Washington, D.C.
| Product Name | Potato Infusion HiVeg™ Agar |
|---|---|
| SKU | MV174 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 1993, Handbook of Microbiological Media, CRC Press, Inc., Boca Raton, |
| Customized Product Available | No |
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