Brucella Agar Base w/ Hemin and Vitamin K

Brucella Agar Base w/ Hemin and Vitamin K is recommended for the isolation, cultivation and subculture of Brucella species and other anaerobes. It is a modified and highly enriched medium. Addition of blood provides nutrients and helps to differentiate hemolytic organisms. Presence of hemin and Vitamin K1 supports growth of other fastidious bacteria like Bacteroides species and gram-positive spore bearers like Clostridium species. The specimen should be inoculated onto the plate as early as possible. Swab cultures are directly streaked. Non-swab cultures are inoculated using an inoculating loop which can be used for the isolation of Brucella and other anaerobic bacteria.