Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Rapid HiColiform™ HiVeg™ Broth
As low as
$50.60
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1453
For detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.
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Rapid HiColiform HiVeg™ Broth is used for detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg special peptone | 5.000 |
| Sodium chloride | 5.000 |
| Sorbitol | 1.000 |
| Dipotassium hydrogen phosphate | 2.700 |
| Potassium dihydrogen phosphate | 2.000 |
| Sodium lauryl sulphate | 0.100 |
| Chromogenic substrate | 0.080 |
| Fluorogenic substrate | 0.050 |
| IPTG (Isopropyl-b-D-thiogalactopyranoside) | 0.100 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.03 grams in 1000 ml distilled water. For double strength broth use 32.06 grams of MV1453 in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle And Interpretation
Rapid HiColiform HiVeg™ Broth is prepared by completely replacing animal based peptones with veg peptones. It is a modification of Rapid HiColiform Broth which is a modification of LMX Broth described by Manafi and Kneifel (2). This medium is useful for the detection and confirmation of Escherichia coli and total coliforms in water samples on the basis of chromogenic and fluorognic substrates (1-6).
The fluorogenic substrate is split by enzyme β-D-glucuronidase specifically found in Escherichia coli. The reaction is indicated by the development of a blue fluorescence under UV light. The presence of total coliforms is indicated by blue-green colouration due to the cleavage of the chromogenic substrate. IPTG amplifies enzyme synthesis and increases the activity of β-D-galactosidase. To confirm presence of E. coli overlay the medium with Kovacs reagent. The layer turns red within 2 minutes in case of positive reaction.
HiVeg Special Peptone, (rich in tryptophan content), provides essential growth nutrients and is useful for the simultaneous detection of indole production. Sorbitol provides the carbon source. The phosphate salts provide buffering action for rapid growth of coliforms. Sodium lauryl sulphate makes the medium selective by inhibiting accompanying microflora, especially the gram-positive organisms.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate
Reaction
Reaction of 1.60% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH
6.60-7.00
Cultural Response
MV1453: Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Medium | Fluorescence (under uv) | Indole production |
|---|---|---|---|---|---|
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant | blue-green | Negative | Negative, no colour development / cloudy ring |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | blue-green | Positive | Positive, red ring at the interface of the medium |
Storage and Shelf Life
Store dehydrated powder and prepared medium at 2-8°C. Use before expiry period on the label.
| Product Name | Rapid HiColiform™ HiVeg™ Broth |
|---|---|
| SKU | MV1453 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Hahn G. and Wittrock E., (1991), Acta Microbiologica Hungarica38(3-4):265-271.2.Manafi. M. and Kneifel W., (1989), Zbl. Hygiene and Umweltmedizin ,,189:225-234.3.Manafi M., (1990), Forum Stadte-Hygiene 41:181-184.4.Manafi M., (1991), Ernahrung / Nutrition, 15, Nr. 10.5.Manafi M. and Kneifel W., (1991), Acta Microbiologica Hungarica 33(3-4):293-304.6.Manafi M., Kneifel B. and Bascon S., (1991), Microbiol. Rev., 55:335-348. |
| Customized Product Available | No |
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