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Shigella HiVeg™ Broth Base
As low as
$238.70
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Availability: In stock
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SKU:
MV1326
For the isolation and cultivation of Shigella species from food.
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Intended Use
Shigella HiVeg Broth Base is used for the cultivation of Shigella species from food.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 20.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 2.0 |
| Monopotassium hydrogen phosphate | 2.0 |
| Dextrose | 1.0 |
| Polysorbate 80 | 1.5 |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 31.5 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 - 50°C and add rehydrated content of 1 vial of Shigella Selective Supplement (FD108) under aseptic conditions. Mix well and dispense in sterile test tubes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the medium free of BSE/TSE risks. Shigella HiVeg Broth Base contains HiVeg hydrolysate as a source of carbon, nitrogen, vitamins and minerals. Dextrose provides carbohydrates. Buffering action in the medium is provided by dipotassium hydrogen phosphate and potassium dihydrogen phosphate. Sodium chloride maintains the osmotic balance of the medium. Polysorbate 80 besides acting as an emulsifier, also to a certain extent serves as a synthetic complex (oleic acid ester), carbon source. Novobiocin is inhibitory towards most gram positive bacteria such as Staphylococcus aureus and certain gram-negative organisms such as Haemophilus influenzae and some species of Proteus.
Technical Data
| Product Type | Code | Description |
|---|---|---|
| Vegetable based (Code MV)© | MV1326 | HiVeg hydrolysate |
| Animal based (Code M) | M1326 | Casein enzymic hydrolysate |
Recommended for: Isolation and cultivation of Shigella species.
Reconstitution: 31.50 g/l
Quantity on preparation (500g): 15.87 L
pH (25°C): 7.0 ± 0.2
Supplement: Shigella Selective Supplement (FD108)
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity: Light amber coloured, clear solution without any precipitate.
Reaction: Reaction of 3.15% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Shigella dysenteriae (13313) | 102-103 | luxuriant |
| Shigella flexneri (12022) | 102-103 | luxuriant |
| Shigella sonnei (25931) | 102-103 | luxuriant |
| Staphylococcus aureus (25923) | 102-103 | inhibited |
| Escherichia coli (25922) | 102-103 | inhibited |
References
- Atlas R.M., 1997, Handbook of Microbiological Media 2nd Edition, CRC Press, Boca Raton, New York, London, Tokyo.
| Product Name | Shigella HiVeg™ Broth Base |
|---|---|
| SKU | MV1326 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Hale T. L., Keusch G. T., 1996, Shigella. In: Barons Medical Microbiology (Barron S et al, Eds.), 4th Ed., Univ of TexasMedical Branch. |
| Customized Product Available | No |
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