Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Medium 11. GN Broth
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$122.10
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SKU:
LQ151
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Intended Use
Recommended for the enrichment of Shigella from pharmaceutical products in accordance with IP 2018.
Composition
| Ingredients | g/L |
|---|---|
| Polypeptone peptone | 20.000 |
| Glucose (Dextrose) | 1.000 |
| Sodium citrate | 2.000 |
| Sodium deoxycholate | 0.500 |
| Di-potassium hydrogen phosphate | 4.000 |
| Potassium dihydrogen phosphate | 1.500 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ151CHL bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle and Interpretation
GN Broth is recommended by the Indian Pharmacopoeia (1) for the selective enrichment of Shigella species with subsequent isolation on a selective medium, XLD Agar (MH031). Croft and Miller isolated more strains of Shigella from rectal swabs using this medium (2). Hajna (3,4) also suggested the enrichment of organisms from rectal swabs in this medium 1-6 hours before plating on solid media.
The medium contains polypeptone peptone, which provides amino acids and other nitrogenous substances to support bacterial growth. The combination of sodium citrate and sodium deoxycholate inhibit gram-positive and some gram-negative bacteria such as coliforms. Phosphates serve as a buffering system. Sodium chloride maintains osmotic equilibrium. Proteus, Pseudomonas and coliforms do not overgrow Salmonella and Shigella in GN Broth during the first 6 hours of incubation. This enrichment broth should be used in conjunction with selective and non selective plating media to increase the probability of isolating pathogens (5,6,7).
Type of Specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear GN Broth in glass bottle.
Colour
Light amber coloured, clear to slightly opalescent solution.
Quantity of medium
10 ml of medium in bottles.
pH
6.80-7.20
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after inoculation in GN Broth and then subculture on XLD Agar and incubation at 30-35°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth in GN broth | Recovery on XLD Agar | Recovery | Colour of colony |
|---|---|---|---|---|---|
| Growth promoting | |||||
| Shigella boydii ATCC 8700 | 50-100 | good | good-luxuriant | >=50% | red translucent |
| Inhibitory | |||||
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 0% | ||
Key (*) - Corresponding WDCM number
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | Medium 11. GN Broth |
|---|---|
| SKU | LQ151 |
| Customized Product Available | No |
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