Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Casitose Yeast Magnesium HiVeg™ Broth (NZYM HiVeg™ Broth)
As low as
$239.80
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1247
For use in the cultivation of recombinant strains of Escherichia coli.
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Recommended for
The cultivation of recombinant strains of Escherichia coli
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1248/MV1247 | M1248/M1247 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Composition
| Ingredients | MV1248 Grams/Litre |
MV1247 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 10.0 | 10.0 |
| Yeast extract | 5.0 | 5.0 |
| Sodium chloride | 5.0 | 5.0 |
| Magnesium sulphate | 0.98 | 0.98 |
| Agar | 15.0 | - |
| Final pH (at 25°C) 7.0 ± 0.2 | ||
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 35.98 grams of MV1248 or 21 grams of MV1247 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by replacing Casein enzymic hydrolysate with HiVeg hydrolysate that makes the medium free of BSE/TSE risks. Casein Yeast Magnesium HiVeg Agar/Broth is a modification of the formula described by Blattner et al (1, 2) used for cultivating recombinant strains of Escherichia coli.
Media constituents like HiVeg hydrolysate and yeast extract supply essential nutrients and cofactors required for excellent growth of recombinant strains of Escherichia coli. Sodium chloride maintains the osmotic balance of the media. Magnesium sulphate is incorporated as a source of magnesium ion necessary in a variety of enzymatic reactions including DNA replication. Agar acts as a solidifying agent and provides substratum for growth of Escherichia coli colonies.
Reconstitution
- (MV1248): 35.98 g/l
- (MV1247): 21.0 g/l
Quantity on preparation (500g)
- (MV1248): 13.89 L
- (MV1247): 23.80 L
pH (25°C)
7.0 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage and Shelf Life
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV1248.
Colour and Clarity
Light to medium amber coloured, slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction
Reaction of 3.59% w/v of MV1248 or 2.1% w/v of MV1247 aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18- 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (23724) | 102-103 | good-luxuriant | >70% |
| Escherichia coli (53868) | 102-103 | good-luxuriant | >70% |
References
- Blattner F.R., Williams B.G., Blechl A.E., et al, 1977, Science, 196:161.
- Sambrook J., Fritsch E. and Manitais T., 1989, Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
| Product Name | Casitose Yeast Magnesium HiVeg™ Broth (NZYM HiVeg™ Broth) |
|---|---|
| SKU | MV1247 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Alcamo E. I., 2001, Fundamentals of Microbiology, 6th Ed., Jones and Bartlett Publishers. |
| Customized Product Available | No |
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