Hanahan’s Broth (SOB Medium)

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M1252
Recommended for use in cultivation of recombinant strains of Escherichia coli.


Hanahan's Broth (SOB Medium) is recommended for use in cultivation of recombinant strains of Escherichia coli that have undergone transformation. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells. Tryptone and yeast extract in the medium supply nitrogenous compounds and growth factors for the recombinant E. coli. Potassium and sodium chloride maintains isotonic conditions. Magnesium sulphate is added to the medium as the necessary component for DNA replication.
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Product Name Hanahan’s Broth (SOB Medium)
SKU M1252
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1.Alcamo I. E., 2001, Fundamentals of Microbiology, 6th Edition, Jones and Bartlett Publishers.2.Sambrook J., Fritsch E. E. and Maniatis T., 1989, Molecular Cloning : A Laboratory Manual, 2nd Ed., Cold Spring Harbor Lab. Press; Cold Spring Harbor, N.Y.3.Hanahan D., 1983, J. Mol. Biol., 166:557.
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