Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Acetate HiVeg™ Agar
As low as
$333.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1225
For the isolation and cultivation of Leuconostoc and Pediococcus species.
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Intended Use
Acetate HiVeg Agar is recommended for the isolation and cultivation of Leuconostoc and Pediococcus species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg extract No. 1 | 5.0 |
| Glucose | 10.0 |
| HiVeg peptone | 5.0 |
| Yeast extract | 5.0 |
| Polysorbate 80 | 0.5 |
| Sodium acetate .3H2O | 27.22 |
| Agar | 20.0 |
| Final pH (at 25°C) | 5.4 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1225 | M1225 |
| HiVeg extract No. 1 | Meat extract |
| HiVeg peptone | Peptic digest of animal tissue |
Recommended for
Isolation and cultivation of Leuconostoc & Pediococcus.
Technical Specifications
| Reconstitution | : 61.9 g/l |
| Quantity on preparation (500g) | : 8.07 L |
| pH (25°C) | : 5.4 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes. |
| Storage | : Dry Medium and Prepared Medium 2 - 8°C. |
Directions
Suspend 61.9 grams of dehydrated powder in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile petri plates.
Principle and Interpretation
Acetate HiVeg Agar is prepared by using HiVeg extract No. 1 and HiVeg peptone which are vegetable based peptones instead of animal based peptones, thus making the medium BSE/TSE risk free. It is the modification of Acetate agar which was formulated by Whittenbury (1) and then modified by Keddie (2) for selective isolation of Lactobacilli. HiVeg peptone, Yeast extract, HiVeg extract No.1 provide all essential growth nutrients. Glucose is the energy source.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 6.19% w/v aqueous solution is pH 5.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Enterococcus faecalis (29212) | none-poor |
| Leuconostoc mesenteroides (12291) | good-luxuriant |
| Pediococcus acidilactici (33314) | good-luxuriant |
References
- Whittenbury R., 1965 b, J. Gen. Microbiol., 40:97.
- Keddie R.M., 1951, Proceed. Soc. Appl. Bacteriol., 14:157.
| Product Name | Acetate HiVeg™ Agar |
|---|---|
| SKU | MV1225 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Vagiakou-Voudris E., Mylona-Petropoulou D., Kalogeropoulou E., Chant zis A., Chini S., Tsiodra P., Malamou-Lada E.,J. Infect. Dis. 2002;34(10):766-7 |
| Customized Product Available | No |
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