main product photo

SA HiVeg™ Agar Base

0 Review
As low as $310.20
Availability: Out of stock
Notify me when the price drops User
Availability: In stock
Only %1 left
SKU:
MV1177
For isolation, cultivation and differentiation of Aeromonas hydrophilia from food based on starch hydrolysis in accordance with APHA.

Intended Use

SA HiVeg Agar Base with Ampicillin Supplement is used for isolation and cultivation of Aeromonas hydrophila from foods.

Product Profile

Vegetable based (Code MV)

Code: MV1177

Description: HiVeg hydrolysate

Animal based (Code M)

Code: M1177

Description: Casein enzymic hydrolysate

Recommended for: Isolation and cultivation of Aeromonas hydrophila from foods.

Composition

Ingredients Grams/Litre
HiVeg hydrolysate 10.0
Sodium chloride 5.0
Starch, soluble 10.0
Phenol red 0.025
Agar 15.0

Final pH (at 25°C): 7.4 ± 0.2

Formula adjusted, standardized to suit performance parameters.

Reconstitution

Quantity on preparation (500g): 12.5 L

pH (25°C): 7.4 ± 0.2

Supplement: Ampicillin Supplement (FD082)

Sterilization: 121°C / 15 minutes

Storage

Dry Medium - Below 30°C, Prepared Medium 2-8°C.

Directions

Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°-50°C. Aseptically add rehydrated contents of 1 vial of Ampicillin Supplement (FD082). Mix well before pouring into sterile petri plates.

Principle and Interpretation

Starch Ampicillin (SA) HiVeg Agar Base is the modification of SA Agar Base which is formulated as described by Palumbo et al (1) and recommended by APHA (2) for isolation and cultivation of Aeromonas hydrophila from foods. This medium is prepared by using vegetable peptone and hence the medium is BSE/TSE risk free. Very few bacteria in food are capable of hydrolyzing starch. Starch hydrolysis is a differentiating characteristic of Aeromonas hydrophila. Typical colonies of Aeromonas hydrophila are yellow to honey coloured surrounded by a clear zone of hydrolyzed starch against black background.

HiVeg hydrolysate provides essential growth nutrients. Sodium chloride maintains osmotic equilibrium. Ampicillin suppresses the contaminating microflora.

SA HiVeg Agar Base is used for quantitative detection of Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae in fresh foods of animal origin and fresh vegetable (3) Aeromonas sobria and Aeromonas caviae are further identified by biochemical tests. Starch hydrolysis is determined by flooding the plate with 5 ml Lugol's Iodine solution.

Quality Control

Appearance of powder: Light pink coloured, homogeneous, free flowing powder.

Gelling: Firm, comparable with 1.5% Agar gel.

Colour and Clarity: Red coloured, clear to slightly opalescent gel forms in petri plates.

Reaction: Reaction of 4.0% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 30°C for 24 - 48 hours.

Organisms (ATCC) Inoculum (CFU) Growth Starch hydrolysis
Aeromonas hydrophila (7966) 102-103 luxuriant +
Escherichia coli (25922) 102-103 poor-fair -
Staphylococcus aureus (25923) 102-103 inhibited -

Key:

  • + = positive reaction, clearing around the colony
  • - = negative reaction, no clearing around the colony

References

  1. Palumbo S., et al, 1985, Appl. Environ. Microbiol., 50:1027.
  2. Frances Pouch Downes and Keith Ito (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
  3. Stern NJ, Drazek ES and Joseph SW 1987, J. Food Protect, 50:66.
More Information
Product Name SA HiVeg™ Agar Base
SKU MV1177
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1. Polumbo S. A., Maxino F., Williams A. C., Buchanan R. L., Thayer D. W., 1985, Appl. Environ. Microbiol., 50:1027.2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.American Public Health Association, Washington, D.C.3.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.
Customized Product Available No
Write Your Own Review
You're reviewing:SA HiVeg™ Agar Base

Related Products