Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
BHI w/6.5% NaCL, HiVeg™ (Brain Heart Infusion w/ 6.5% NaCl, HiVeg™)
As low as
$256.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1037
For selective cultivation of salt tolerant microorganisms.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ special infusion | 7.5000 |
| HiVeg™ infusion | 10.000 |
| HiVeg™ peptone No. 3 | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 65.000 |
| Disodium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 97.0 grams in 1000 ml purified/distilled water. Dispense into tubes or flasks as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.
Principle And Interpretation
Brain Heart Infusion Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1).
BHI w/6.5% NaCL, HiVeg™ is employed for the selective cultivation of salt tolerant microorganisms. High concentration of sodium chloride acts as a differential and/or selective agent by interfering with membrane permeability and osmotic and electro kinetic equilibrium in salt intolerant organisms. BHI w/6.5% NaCL, HiVeg™ is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones.
HiVeg™ special infusion and HiVeg™ infusionserve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium.
Type of specimen
Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
- Heavy inoculation may result into errorneous results.
- Infusion broth with 6.5% NaCl may produce slow reactions thereby making test interpretation difficult.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to light yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured, clear solution without any precipitate
Reaction
Reaction of 9.7% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | >=104 | inhibited |
| Streptococcus pneumoniae ATCC 6303 | >=104 | inhibited |
| Streptococcus pyogenes ATCC 19615 | >=104 | inhibited |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCMnumbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | BHI w/6.5% NaCL, HiVeg™ (Brain Heart Infusion w/ 6.5% NaCl, HiVeg™) |
|---|---|
| SKU | MV1037 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Rosenow, 1919, J. Dental Research, 1:205. |
| Customized Product Available | No |
Write Your Own Review
BHI w/ 6.5% NaCL (Brain Heart Infusion w/ 6.5% NaCl)
As low as
$256.30
Upsell Products
HiEncap™ BHI Broth
As low as
$0.00
BHI Broth
As low as
$37.40