Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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BHI w/ 0.1% Agar, HiVeg™ (Brain Heart Infusion with 0.1% Agar, HiVeg™)
As low as
$262.90
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1036
For propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.
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Intended Use
Recommended for propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ special infusion | 7.5000 |
| HiVeg™ infusion | 10.000 |
| HiVeg™ peptone No. 3 | 10.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Dextrose (Glucose) | 2.000 |
| Agar | 1.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 38.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense into bottles or tubes as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.
Principle And Interpretation
BHI w/ 0.1% Agar, HiVeg™ is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). Brain Heart Infusion Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3,4). This medium is nutritious and well buffered to support the growth of wide variety of organisms (3,5,6). BHI w/ 0.1% Agar, HiVeg™ is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (7) and other fungi. Agar in 0.1% concentration improves growth of microaerophillic and anaerobic microorganisms (3). For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended (8).
HiVeg™ special infusion, HiVeg™ infusion and HiVeg™ peptone No. 3 serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium hydrogen phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium. Agar in 0.1% concentration helps create appropriate conditions for growth of anaerobic bacteria.
Type of specimen
Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitation
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- This medium with added 10% sheep blood, gentamicin and chloramphenicol is inhibitory to certain fung
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to light yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light to medium amber coloured, clear solution without any precipitate
Reaction
Reaction of 3.8% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | BHI w/ 0.1% Agar, HiVeg™ (Brain Heart Infusion with 0.1% Agar, HiVeg™) |
|---|---|
| SKU | MV1036 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Rosenow, 1919, J. Dental Research, 1:205. |
| Customized Product Available | No |
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BHI w/0.1% Agar (Brain Heart Infusion w/ 0.1% Agar)
As low as
$262.90
