Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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BHI HiCynth™ Broth (Brain Heart Infusion HiCynth™ Broth)
As low as
$41.80
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MCD210
Recommended for propagation of pathogenic cocci and other fastidious organisms associated with blood culture work and allied pathological investigations.
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Intended Use
It is employed for the propagation of pathogenic cocci other fastidious organisms associated with blood culture work and allied pathological investigations.
Composition
| Ingredients | g/L |
|---|---|
| HiCynth™ Peptone No.2* | 8.000 |
| HiCynth™ Peptone No.3* | 9.500 |
| HiCynth™ Peptone No.6* | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
* Chemically defined peptones.
Directions
Suspend 37.0 gram in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled to 45-50°C before use.
Principle And Interpretation
BHI HiCynth™ Broth is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3,11). BHI HiCynth™ Medium is prepared by completely replacing animal or vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones. This medium is nutritious and well buffered to support the growth of wide variety of organisms (2,5,6). With the addition of 10%defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (7) and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended (8).
HiCynth™ Peptone No.2, HiCynth™ Peptone No.3 and HiCynth™ Peptone No.6 serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium hydrogen phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Food and dairy samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,9,10). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- As organisms differ in their nutritional requirements, some fastidious organisms may be inhibited or may show poor growth.
- Biochemical and serological tests must be performed for confirmation of isolated organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light to medium amber coloured, clear solution without any precipitate
Reaction
Reaction of 3.7% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (11,12).
| Product Name | BHI HiCynth™ Broth (Brain Heart Infusion HiCynth™ Broth) |
|---|---|
| SKU | MCD210 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Rosenow, 1919, J. Dental Research, 1:205. 2.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. 3.Atlas R. M., 1993, Handbook of Microbiological Media, 147-153, CRC Press, Boca Raton, FL. 4.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 5.Roseburg T. et al, 1944, J. Inf. Dis., 74:131 6.Conant N. F., 1950, Diagnostic Procedures and Reagents, 3rd Ed., APHA Inc., New York 8.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 9.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C. 10.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc.,Washington, D.C |
| Customized Product Available | No |
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