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HiCrome™ Strep B Selective HiCynth™ Agar Base
As low as
$73.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MCD1840
Recommended for selective isolation of Group B Streptococci from clinical samples.
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Intended use
HiCrome™ Strep B Selective GlBxnsg|| Agar Base is recommended for selective isolation of Group B streptococci from clinical samples.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| HiCynth™ peptone No.1* | 11.000 |
| HiCynth™ peptone No.6* | 6.500 |
| Buffers | 2.500 |
| Chromogenic mixture | 2.540 |
| Selective agents | 0.110 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
* Chemically defibed peptones
Directions
Suspend 37.65 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add the rehydrated contents of one vial of Hicrome™ Strep B Selective Supplement (FD273). Mix well and pour into sterile Petri plates.
Principle And Interpretation
Group B Streptococcus is a leading infection causing illness and death in newborns. Group B streptococci can also cause serious diseases in pregnant women, the elderly, and adults with other illnesses. GBS normally reside in the vagina of women and rectum of men and women (1). In newborns, group B strep is the most common cause of sepsis (infection of the bloodstream) and meningitis (infection of the lining and fluid surrounding the brain) and a common cause of pneumonia. In adults, group B strep can rarely lead to serious bloodstream infections, urinary tract infections, skin infections, and pneumonia, especially in people with weak immune systems. Heavy colonization of the maternal genital tract is associated with colonization of infants and risk of neonatal disease (2).
HiCrome™ Strep B Selective HiCynth™ Agar Base is preparred by completely replacing animal based peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.
The sample collection is usually done by collection of vaginal and rectal swab between 35 and 37weeks of pregnancy. The swab is then processed on HiCrome™ Strep B Selective HiCynth™ Agar Base. For the conventional methods optimum recovery is however achieved by selective enrichment into Todd Hewitt broth with colistin and nalidixic acid and then subculture on Blood Agar (3,4).
HiCynth™ peptone No.1 and HiCynth™ peptone No.6 provides nitrogeneous and carbonaceous compounds, long chain amino acids and other essential nutrients for the growth of Streptococci. Buffers present provides buffering to the medium. Selective agents in the medium inhibits accompanying flora. One of the substrate in the chromogenic mixture is cleaved by beta glucosidase possesed by Group B Streptococci resulting in blue coloured colonies.
Type of specimen
Clinical samples - Vaginal and rectal samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidleines must be followed while handling clincal specimens. Saftey guidelines may be referred in individual safety data sheets
Limitations :
Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured opaque gel forms in Petri plates
Reaction
Reaction of 3.77% w/v aqueous solution at 25°C. pH : 7.3±0.2
Cultural Response
Cultural characteristics observed with added Hicrome Strep B Selective Supplement (FD273), after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=10³ | inhibited | 0% | - |
| Neisseria meningitidis ATCC 13090 | >=10³ | inhibited | 0% | - |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=10³ | inhibited | 0% | - |
| Streptococcus agalactiae ATCC 13813 | 50-100 | luxuriant | >=50% | blue |
*- Corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | HiCrome™ Strep B Selective HiCynth™ Agar Base |
|---|---|
| SKU | MCD1840 |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.Anthony BF, Okada DM, Hobel CJ. Epidemiology of group B Streptoccoccus: longitudinal observations during pegnancy.J.Infect Dis 1978; 137:524-30.2.Murray P.R. , Baron J.H., Manual of Clinical Microbiology Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H.and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington,D.C.3.Prevention of perinatal group B Streptococcal disease: a public health perspective . Centres for Disease control andPrevention. MMWR Recomm Rep 1996; 51:1-22HiMedia Laboratories Technical Data4.NHS Processing swabs for Group B Streptococcal carriage Issue no.2.1,2006 |
| Customized Product Available | No |
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