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HiCrome™ MM HiCynth™ Agar, Modified (Hicrome™ Miller and Mallinson HiCynth™ Agar)
As low as
$57.20
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MCD1816
Recommended for identification and differentiation of Salmonella and non Salmonella like Citrobacter from water samples and clinical samples.
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Intended use
Recommended for identification and differentiation of Salmonella and non Salmonella like Citrobacter from water samples.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ peptone No.2# | 6.000 |
| HiCynth™ peptone No.5# | 10.000 |
| L-Lysine hydrochloride | 5.000 |
| D-Cellobiose | 10.000 |
| Lactose | 10.000 |
| Sucrose | 10.000 |
| D-Xylose | 3.750 |
| Ferric ammonium citrate | 0.800 |
| Sodium thiosulphate | 6.800 |
| Chromogenic mixture | 0.200 |
| Bromothymol blue | 0.100 |
| Agar | 18.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Chemically defined peptones
Directions
Suspend 80.65 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
HiCrome™ MM Agar was formulated by Miller and Mallison (1) for specific isolation and detection of Salmonellae. This medium is superior to XLT4 Agar in supporting growth of Salmonella due to the presence of appropriate proportion of four sugars. HiCrome™ MM Agar, Modified is a slight modification of HiCrome™ MM Agar and designed to differentiate Enterobacteriaceae especially Salmonella from Proteus and Citrobacter group. The utilization of sugars by organisms results in pH-changes. This is used as a means of distinguishing Salmonella from competing bacteria on the basis of colony colour.
Salmonella are gram negative, anaerobic, non sporulating rods in the family Enterobacteriaceae present in the stomach and intestinal tissues of human & animals and are found in their wastes. Salmonella usually are unable to ferment the sugars (2) that support growth of competing bacteria. Thus other bacteria tend to overgrow Salmonellae, masking their presence. HiCrome™ MM HiCynth™ Agar Modified is prepared by completely replacing animal based peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.
HiCynth™ peptone No.2 and HiCynth™ peptone No.5 is a source of carbon, nitrogen and other essential amino acid and growth factor. To add to the differentiating ability of the formulation, an H2S indicator system, consisting of sodium thiosulphate and ferric ammonium citrate, is included for the visualization of hydrogen sulphide produced, resulting in the formation of colonies with black centers. Bromothymol blue act as a pH indicator. The inclusion of sugars like lactose, sucrose, xylose and cellobiose provides source of fermentable carbohydrate which stimulate the better initial growth of Salmonella cells. Presence of lactose suppresses H2S production by non-salmonellae like Citrobacter freundii. A chromogenic mixture, present in this medium helps to differentiate between lactose fermenters and nonfermenters. Lactose fermenters give bluish green coloured colonies, which would have been impossible to differentiate with an indicator based on pH change.
Type of specimen
Water samples; Food samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection and processing as per guidelines (3).
For food samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards(4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Though most of the Salmonella produce H2S certain non H2S producing Salmonella species may appear as colourless colonies.
- Certain Salmonella species which are lactose fermenters may show as bluish green coloured colonies
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further confirmation may be carried out on suspected colonies.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.8% Agar gel
Colour and Clarity of prepared medium: Bluish green coloured, clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 8.07% w/v aqueous solution at 25°C. pH: 7.6±0.2
pH: 7.40-7.80
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Citrobacter freundii ATCC 8090 | 50-100 | good-luxuriant | >=50% | Yellow coloured |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% | Bluish green |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | black centered |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | black centered with yellow zone |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | Black centered |
| Proteus mirabilis ATCC 25933 | 50-100 | good-luxuriant | >=50% | Gray coloured |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | luxuriant | >=50% | Yellowish green |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiCrome™ MM HiCynth™ Agar, Modified (Hicrome™ Miller and Mallinson HiCynth™ Agar) |
|---|---|
| SKU | MCD1816 |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.Miller R.G. and Mallison E.T., 2000, J. Food Protection, 63(10), 1443-46.2.Miller R.G., Tate C.R., Mallinson E.T. and Scherrer J.A., 1991, Pault Sa 70:2429-32. |
| Customized Product Available | No |
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