Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
HiCrome™ Enrichment HiCynth™ Broth Base for ECO157:H7
As low as
$294.80
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MCD1598
Recommended for isolation and selective differentiation of Escherichia coli O157:H7 from food, environmental and clinical samples by chromogenic method.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for isolation and selective differentiation of Escherichia coli O157:H7 from food and environmental samples by chromogenic method.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ peptone No.1# | 10.000 |
| Sorbitol | 10.000 |
| Synthetic detergent No.1 | 1.500 |
| Chromogenic mixture | 1.300 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 11.4 grams in 500 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. For selective isolation of E.coli O157:H7, aseptically add the rehydrated contents of 1vial of NPt Selective Supplement I (FD230) to sterile, molten and cooled (at 45-50°C) medium. Mix well and dispense into sterile test tubes.
Principle And Interpretation
March and Ratnam (1) reported the inability of Escherichia coli O157:H7 to ferment sorbitol while developing Sorbitol MacConkey medium. Subsequently Thomson et al (2) observed the absence of ß-glucuronidase activity in E.coli O157:H7 from a variety of samples by direct culture. HiCrome™ Enrichment HiCynth™ Broth Base for EC O157:H7 is a modification of HiCrome™ Enrichment Broth Base for EC O157:H7 wherein animal based peptones are completely replaced with chemically defined peptones to avoid BSE/TSE/ GMO risks associated with animal peptones. The medium contains HiCynth™ peptone No.1 that provides nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Sorbitol is the fermentable carbohydrate. Synthetic detergent No.1 inhibits most of the gram-positive organisms. Addition of NPt Selective Supplement (FD230) makes the medium more specific and selective. The bluish colour development by colonies of E.coli and Klebsiella in the medium is due to the enzymes B-glucuronidase and B-D-galactosidase that cleaves the chromogenic substrates present in chromogenic mixture respectively. However E.coli O157:H7 gives a purple colour to the medium due to the absence of B-glucuronidase and its inability to ferment sorbitol.
Type of specimen
Food samples.
Specimen Collection and Handling
For food samples follow appropriate techniques for sample collection and processing as per guidelines (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical or serological testing can be done for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of Prepared Medium
Light yellow coloured, clear solution which may have slight precipitate.
Reaction
Reaction of 2.28% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed with added NPt Selective Supplement(FD230), after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Medium | Growth (after addition of FD230 | Colour of Medium (after addition of FD230 |
|---|---|---|---|---|---|
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | good-luxuriant | bluish-green may show slight precipitation of growth | good | bluish-green may show slight precipitation of growth |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | blue may show slight precipitation of growth | inhibited | - |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | - | inhibited | - |
| Escherichia coli 0157:H7 (NCTC 12900) (00014*) | good-luxuriant | purple may show slight precipitation of growth | good-luxuriant | purple may show slight precipitation of growth | |
| #Cronobacter sakazakii ATCC 12868 | 50-100 | good-luxuriant | white may show slight precipitation of growth | none-poor | colourless may show slight precipitation of growth |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | - | inhibited | - |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | light green may show slight precipitation of growth | good | light green may show slight precipitation of growth |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | good | colourless | inhibited | - |
Key: (*) Corresponding WDCM numbers, (#) Formerly known as Enterobacter sakazakii.
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
| Product Name | HiCrome™ Enrichment HiCynth™ Broth Base for ECO157:H7 |
|---|---|
| SKU | MCD1598 |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.March S. B and Ratnam S., 1986, J. Clin. Microbiol., 23:869-872.2.Thompson J. S., Hodge D. S., Borczyk A. A., 1990, J. Clin. Microbiol.28, 2165-2168.3.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. |
| Customized Product Available | No |
Write Your Own Review
HiCrome™ Enrichment Broth Base for EC O157 : H7
As low as
$268.40
Upsell Products
Hiaureus™ Coagulase Confirmation Kit (w/o swabs)
As low as
$0.00
Bile Esculin Agar Plate
As low as
$37.40
Bile Esculin Azide Agar Plate
As low as
$163.90
Baird Parker Agar Plate
As low as
$72.60
Bile Esculin Azide Medium w/ Sterile Membrane Filter
As low as
$259.60
HiMotility™ Biochemical Kit for Listeria
As low as
$0.00
HiMotility™ Biochemical Kit for Salmonella
As low as
$0.00
HiMotility™ Biochemical Kit for E.coli
As low as
$0.00
Bile Esculin Agar Slant in Tubes
As low as
$41.80
Biochemical Test Kit for Salmonella
As low as
$39.60
Biochemical Test Kit for Vibrio parahaemolyticus
As low as
$139.70
Biochemical Test Kit for Vibrio cholerae
As low as
$139.70
HiListeria™ Identification Kit
As low as
$0.00
HiSalmonella™ Identification Kit
As low as
$0.00
HiE. coli™ Identification Kit
As low as
$0.00
HiVibrio™ Identification Kit
As low as
$0.00
HiStaph™ Identification Kit
As low as
$0.00
Bile Esculin Agar
As low as
$49.50
HiCrome™ Salmonella Agar
As low as
$0.00