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HiCrome™ M-TEC HiCynth™ Agar
As low as
$89.10
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Availability: In stock
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SKU:
MCD1571
Recommended for differentiation and enumeration of thermotolerant Escherichia coli in water by the membrane filtration technique.
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Intended Use
Recommended by the U.S. Environmental Protection Agency (USEPA) for differentiation and enumeration of thermotolerant Escherichia coli in water by the membrane filtration technique. It can also be used for clinical samples.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.3* | 5.000 |
| HiCynth™ Peptone No.5* | 3.000 |
| Lactose | 10.000 |
| Sodium chloride | 7.500 |
| Dipotassium hydrogen phosphate | 3.300 |
| Potassium dihydrogen phosphate | 1.000 |
| Sodium lauryl sulphate (SLS) | 0.200 |
| Synthetic detergent I | 0.100 |
| Chromogen | 0.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
* Chemically defined peptones
Directions
Suspend 45.6 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
HiCrome™ M-TEC Agar is a chromogenic media used for detection and enumeration of thermo-tolerant E.coli (TEC)in water by membrane filtration (5), developed by Dufour (2). The modified medium contains the chromogen, 5-bromo-6-chloro-3-indolyl-ẞ-D-glucuronide that is cleaved by enzyme ẞ-D-glucuronidase to yield glucuronic acid, produced by E.coli strains. This imparts a purple-magenta colour to the colonies of E. coli only. E.coli can be isolated from clinical samples. HiCrome™ M-TEC HiCynth™ Agar is the modification of the same using chemically defined peptones instead of vegetable and animal peptones to avoid BSE/TSE/GMO risks. HiCynth™ Peptone No.3 and HiCynth™ Peptone No.5 provides carbon and nitrogen compounds, amino acids, vitamins and essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Potassium dihydrogen phosphate and dipotassium hydrogen phosphate provide strong buffering system to control the pH in the presence of fermentative action. Sodium lauryl sulphate and sodium deoxycholate make the medium more selective by inhibiting gram positive bacteria. Membrane filter through which water sample has been passed is aseptically placed on the medium. The plates are then incubated at 44.5±0.2°C for 22-24 hours. Following incubation E.coli will form purple to magenta coloured colonies on the membrane filters.
Type of specimen
Clinical samples-urine, Water samples.
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical test must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm,comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.56% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 44.5±0.2°C for 22-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Colony |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good to luxuriant | purple/ magenta |
| Enterococcus faecalis ATCC >=104 29212 (00087*) | inhibited | ||
| Proteus mirabilis ATCC 25933 | 50-100 | good | colourless-light brown |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | good | tan-light purple |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | HiCrome™ M-TEC HiCynth™ Agar |
|---|---|
| SKU | MCD1571 |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.U.S. Environmental Protection Agency, 2002, Method 1603; Publication EPA-821-R-02-023.2.Dufour,Strickland and Cabelli, 1981, Appl. Environ. Microbiol. 41: 1152.3.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rd ed., APHA, Washington, D.C 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock.,D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1 |
| Customized Product Available | No |
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