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HiCrome™ Improved Salmonella HiCynth™ Agar
As low as
$64.90
Availability: Out of stock
Availability: In stock
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SKU:
MCD1466
Improved selective and differential medium for Salmonella species from clinical and non clinical samples.
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Intended use
Recommended as an improved selective and differential medium for Salmonella species.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.3* | 8.000 |
| HiCynth™ Peptone No.5* | 2.000 |
| Synthetic detergent | 1.000 |
| Chromogenic mixture | 3.250 |
| Agar | 12.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 26.25 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Salmonella species have been isolated from humans and almost all animals throughout the world. They cause many types of infections from mild, self-limiting gastroenteritis to life threatening typhoid fever. Salmonella Typhi and Salmonella Paratyphi A & B cause gastroenteritis, bacteremia and enteric fever, Salmonella Choleraesuis causes gastroenteritis and enteric fever, especially in children. Salmonella Typhimurium is the most frequently isolated serotype of Salmonella (1).
HiCrome™ Improved Salmonella Agar is a modification of the original formulation of Rambach (2) and is used for the differentiation of Salmonella species from other enteric bacteria. Rambach formulation differentiates Salmonella based on propylene glycol utilization and presence of a chromogenic indicator. However, HiCrome™ Improved Salmonella HiCynth™ Agar uses only a chromogenic mixture which contains chromogenic substrate and indicator dye for identification and differentiation of Salmonella species. It is prepared by completely replacing animal based peptone and vegetable peptone with chemically defined peptone to avoid BSE/TSE risks associated with animal peptones.
HiCynth™ Peptone No.3 and HiCynth™ Peptone No.5 provides nitrogenous, carbonaceous compounds, long chain amino acids and other essential growth nutrients. Escherichia coli and Salmonella are easily distinguishable due to their colony characteristics.
All Salmonella species isolated from food or clinical sample exhibit pink to red colonies including Salmonella Typhi. E. coli exhibits a characteristic blue to purple colour, due to presence of the enzyme specific for chromogenic substrate. Synthetic detergent inhibits gram-positive organisms.
Type of specimen
Food samples; Water samples.
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The medium is selective for Salmonella may not support the growth of other microorganisms.
- Most of the Salmonella strains show pink-red colonies except few which may show colorless colonies.
- Due to nutritional variations, some strains may show poor growth.
- Final confirmation of suspected colonies must be carried out by serological and biochemical tests.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Reddish pink coloured, slightly opalescent gel forms in Petri plates
Reaction
Reaction of 2.62% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% | green to blue |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | pink to red |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | pink to red |
| Proteus vulgaris ATCC 13315 | 50-100 | good | 40-50% | light brown |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | light pink |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiCrome™ Improved Salmonella HiCynth™ Agar |
|---|---|
| SKU | MCD1466 |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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