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HiCrome™ MacConkey Sorbitol HiCynth™ Agar Base

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MCD1340

Used for selective isolation of Escherichia coli O157 : H7 from food and animal feeding stuffs.

Description

Recommended for the detection of enteropathogenic strains of Escherichia coli O157:H7 that ferments lactose but does not ferment sorbitol and hence produce colourless colonies. Sorbitol fermenting strains of Escherichia coli produce pink-red colonies. The red colour is due to production of acid from sorbitol, absorption of neutral red and a subsequent colour change of the dye when pH of the medium falls below 6.8. Sorbitol positive and beta glucuronidase positive E.coli appears as Blue to purple colour colony. Tellurite-cefixime supplement imparts selectivity.

Product Information

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Product Name	HiCrome™ MacConkey Sorbitol HiCynth™ Agar Base
SKU	MCD1340
Product Type	HiCynth™, HiCrome™
Physical Form	Powder
Origin	Chemically defined (HiCynth™)
Packaging type	HDPE
References	1. Hansen W. and Yourassawsky E., 1984, J. Clin. Microbiol., 20:1177.2.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.3.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual ofClinical Microbiology, 11th Edition. Vol. 1.4.Karmali M.A., Petric M., Lim C., et al, 1985, J. Infect. Dis.151-775.5.March S.B. and Ratnam S. (1986): J. Clin. Microbiol. 23,869-872.6.Rappaport F. and Henigh E., 1952, J. Clin. Path., 5:361.7.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2001, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.8.Thompson et al (1990). J. Clin. Microbiol. 29, 2165-2168.9.Zadik P.M., Chapman P.A., and Siddons C.A. (1993), J. Med. Microbiol.39, 155-158.
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