Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Glucose Azide Broth
As low as
$236.50
Availability: Out of stock
Availability: In stock
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SKU:
M982
Used for the enumeration of faecal Streptococci by MPN technique from water and sewage.
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Intended Use
Recommended for the enumeration of faecal Streptococci by MPN technique from water and sewage.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 10.000 |
| Yeast extract | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 5.000 |
| Potassium dihydrogen phosphate | 2.000 |
| Dextrose (Glucose) | 5.000 |
| Sodium azide | 0.250 |
| Bromo cresol purple | 0.030 |
| Final pH (at 25°C) | 6.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.28 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense 5ml amounts in 16 x150 mm test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For large inocula of 5 ml or more quantities, prepare double strength medium.
Principle And Interpretation
Fecal Streptococcus is a group of bacteria normally present in large numbers in the intestinal tract of warm-blooded animals other than human. The fecal Streptococcus group consists of a number of species of the genus Streptococcus such as Streptococcus faecalis, Streptococcus faecium, Streptococcus avium, Streptococcus bovis, Streptococcus equines and others. They have been used with fecal coliforms to differentiate fecal contamination from humans and from that of other warm-blooded animals. They tend to persist longer in the environment than faecal coliforms. Glucose Azide Broth is recommended by Hannay and Norton (3) for enumeration of faecal streptococci by MPN technique from water, sewage, foods and other materials suspected to be contaminated with sewage. Glucose Azide Broth is a highly nutritious medium due to its content of peptone, yeast extract and dextrose, which provide nitrogenous compounds, carbon, sulphur, amino acids and trace ingredients. Sodium chloride maintains osmotic balance of the medium. Sodium azide suppresses the growth of gram-negative organisms and thereby allows the selective growth of faecal Streptococci.
Type of specimen
Water sample
Specimen Collection and Handling:
Prior to inoculation, warm the tubes of Glucose Azide Broth to 44-45°C by heating in a water bath. Inoculate the tubes containing Glucose Azide Broth with heavy inocula from all the positive presumptive test tubes. MacConkey Broth purple (M083) is generally used for the presumptive test. Incubate inoculated Glucose Azide Broth tubes at 44-45°C for 24-48 hours. Tubes showing yellow colour change, due to acid production are subcultured onto Bile Esculin Azide Agar (M493) for confirming the presence of faecal streptococci (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. The media should be handled by trained personnel only. Ear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temmperature.
Quality Control
Appearance: Light yellow to beige homogeneous free flowing powder
Colour and Clarity of prepared medium: Purple coloured, clear solution without any precipitate
Reaction: Reaction of 3.03% w/v aqueous solution at 25°C. pH : 6.7±0.2
pH: 6.50-6.90
Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour change to yellow |
|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant | positive |
| Enterococcus hirae ATCC 8043 (00089*) | 50-100 | good-luxuriant | positive |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | negative |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | negative |
Key: *- Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with with current laboratory techniques (4,5).
Reference
- Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rd ed., APHA, Washington, D.C.
- Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P., (Eds) Mackie and McCartney, Practical Medical Microbiology, 1989, 13th Edition, Churchill Livingstone
- Hannay C. L., Norton I. L., 1947, Proc. Soc. Appl. Bacteriol.1: 59
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Glucose Azide Broth |
|---|---|
| SKU | M982 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Hannay C. L., Norton I. L., 1947, Proc. Soc. Appl. Bacteriol.1: 592.Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P., (Eds) Mackie and McCartney, Practical Medical Microbiology,1989, 13th Edition, Churchill Livingstone |
| Customized Product Available | No |
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