Asparagine Broth for Pseudomonas is recommended for the presumptive identification and enumeration of Pseudomonas by MPN method.It is an enrichment broth for Pseudomonas aeruginosa. The composition is strictly mineral base with Asparagine as the sole source of nitrogen. The Potassium salts act as a buffer system and Magnesium sulfate is a magnesium ion required in a large variety of enzymatic reactions, including DNA replication and also acts as a buffer.