PE-2 Medium is recommended for detection and cultivation of mesophilic anaerobic spore formers in specimens collected from food processing plants. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex and trace ingredients required for the growth of clostridia. Addition of untreated alaska seed peas creates anaerobic conditions in the medium. Clostridium botulinum, Clostridium sporogenes and Thermoanaerobacterium thermosaccharolyticum grows luxuriantlly on this media.
1. Folinazzo J. F. and Troy V. S., 1954, Food Technol., 8:280. 2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.