Tryptose Cycloserine Dextrose Agar Base is recommended for the isolation of mesophilic spore forming anaerobes in food spoilage as per APHA. This medium is used for isolation of mesophilic spore forming anaerobes in food spoilage. It has been effectively used as selective media for the isolation and enumeration of mesophilic anaerobic spore formers from environmental samples collected from cannery plant surveys. Incorporation of D-cycloserine in this medium effectively inhibits growth of most Enterococci.
1. Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.2.Lake D. E., Leseniewski R. S., Anderson J. E., Graves R. R. and Bremser J. F., 1985, J. Food Prot. 48: 794.