Lee’s Agar is used for differential enumerations of yoghurt starter bacteria ( Lactobacillus bulgaricus and Streptococcus thermophilus ).This medium described by Lee et al is used for the differential enumeration of yoghurt starter bacteria. This medium is also recommended by APHA for the same purpose. It contains sucrose, which most L. bulgaricus strains will not ferment, but S. thermophilus will, and lactose, which both species utilize. Bromocresol purple is the pH indicator, which turns yellow in acidic condition and imparts yellow colour to the colony.Lactobacillus bulgaricus shows luxuriant growth on media with white colored colony; while Streptococcus thermophilus grows luxuriantlly giving yellow colored colony.
1. Lee S. Y., Vedamuthu E. R., Washam C. J. and Reinbold G. W., 1974, J . Milk Food Technol., 37: 272 2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. 3.Davis J. G., Ashton T. F. and MaCaskill M., 1971, Dairy Ind., 36:569.