Kupferberg Trichomonas Broth with added enrichments and antibiotics is used for selective isolation and cultivation of Trichomonas species. The medium contains Tryptone, which provides the nitrogenous substances required for growth. Maltose acts as energy source. The selective agents Streptomycin or chloramphenicol and penicillin are inhibitory to accompanying gram-positive and gram-negative bacteria but not to Trichomonas species