Trichomonas Broth Base No. 2 is recommended for the isolation of Trichomonas vaginalis. Addition of Horse serum makes the medium highly nutritious for T. vaginalis. The medium is equally suitable for the examination of urethral and vaginal swabs and urine specimens. The selective agent chloramphenicol is inhibitory to gram-positive and gram-negative bacteria but not for Trichomonas species.