Mycoplasma Broth Base w/ CV (PPLO Broth Base w/ CV) is used in a study of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms. Crystal violet and potassium tellurite inhibits many gram-negative and gram positive bacteria.