PPLO Broth Base w/o CV (Mycoplasma Broth Base w/o CV) with added enrichment supplement and horse serum or ascitic fluid is used for isolation and cultivation of Mycoplasma species (pleuropneumonia like organisms). This medium does not contain crystal violet. Most of the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Broth cultures on subculturing onto PPLO Agar, produce colonies exhibiting the typical morphology, i.e., fried egg appearance.