Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Pseudomonas Medium (without Membrane Filter)
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$66.00
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SKU:
MF023
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Intended Use
Recommended for detection and enumeration of Pseudomonas species.
Directions
The test sample should be filtered through a sterile membrane filter having pore size of 0.45µ. Rehydrate the nutrient pad with 2.0-2.5 ml sterile distilled / purified water. After filtration, remove the membrane filter aseptically using sterile forceps. Place the membrane filter on rehydrated nutrient pad. Incubate the inoculated nutrient pad. Interpret the results qualitatively by observing the presence or absence of growth and quantitatively by counting the number of colonies on the surface of the membrane filter and calculating CFU/ml.
Principle And Interpretation
DriFilter Membrane Nutrient Pad Medium is ready to use sterile culture media in the form of a 50 mm biological inert absorbent pads impregnated with Pseudomonas Medium, then dried and sterilized in 55 mm Petri plate. They eliminate the need of laborious media preparation and autoclaving procedures. The nutrient pads are to be just rewetted with sterile distilled water and are ready to use. Use of nutrient pads allows larger sample volumes to be tested at a time. Interpretation of results is directly by counting the CFUs and also quantifies the microbial load present in the sample. Pseudomonas Agar is based on the formulation described by King et al (3).
A pyocyanin-producing Pseudomonas strain will usually also produce fluorescein. It must, therefore, be differentiated from other simple fluorescent Pseudomonads by other means. Temperature can be a determining factor as most other fluorescent strains will not grow at 35°C. Rather, they grow at 25-30°C (4).
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be performed for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Dry filter membrane pad of 50mm diameter
Colour
Light yellow coloured nutrient pad
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristicis observed after an incubation at 25-30°C for 24 - 48 hours
| Organism | Inoculum (CFU) | Growth | Colour of Medium |
|---|---|---|---|
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | Green |
| ## Proteus hauseri ATCC 13315 | 50-100 | inhibited | - |
Key: (*) Corresponding WDCM numbers, ## Formerly known as Proteus vulgaris
Storage and Shelf Life
On receipt store between 10-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Pseudomonas Medium (without Membrane Filter) |
|---|---|
| SKU | MF023 |
| Customized Product Available | No |
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