L.mono Blood Agar Base is recommended for the specific isolation and cultivation of Listeria species from food and environmental samples. It uses Tryptone Soya Agar as a base with the addition of lithium chloride as a selective agent. This medium with the addition of 5% w/v sterile defibrinated sheep blood helps in the differentiation of haemolytic and pathogenic Listeria species which includes L. monocytogenes, L. seeligeri and L. ivanovii from non-haemolytic and non-pathogenic species which include L. innocua, L. grayi and L. welshimeri.