BAT Medium is used for the isolation of Alicyclobacillus species from fruit juices. BAT Medium has a pH of 4.0 ± 0.2 which supports the growth of Alicyclobacillus species and inhibits most of the microbial flora. Rest of the microbial flora is inhibited at 60°C, which is the optimum growth temperature for Alicyclobacillus species.