This medium is prepared as per the British Pharmacopoeia and is cited as Medium R. It is useful in semi-quantitative test for presence of C. perfringens in pharmaceutical products where the level of this species is a criterion of quality. Sodium metabisulphite and ferric ammonium citrate act as indicators of sulphite reduction, indicated by blackening of the medium.
1. European Pharmacopoeia, 2002, Suppl.4.2.(2001). Chp. 2.6.13, 4th Ed., Council of Europe, Strasbourg2.British Pharmacopoeia, 2004, The Stationery office British Pharmacopoeia.3.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Infect. Immun., 61: 3429-3439.4.International Organization for Standardization (ISO), 1997, Draft ISO/DIS 7937:1997.